Lab Update 29 – New Comprehensive Solid Tumour Panel: Oncomine™ Precision Assay

March 2022
Ampath | ampath.co.za

Overview

Tumour genetic profiling is essential for:

• Confirming tumour diagnosis
• Determining prognosis
• Guiding precision therapy

The aim:

Deliver the right treatment to the right patient at the right time through precision oncology, thereby improving cancer outcomes.

Introducing the Oncomine™ Precision Assay

Ampath now offers the Oncomine™ Precision Assay, a next-generation sequencing (NGS) solution designed for comprehensive solid tumour profiling.

Key features:

• Expanded gene coverage compared to the older EGFRASSEQ panel
• Detects 2,769 unique variants
• Includes:
  • 45 mutations
  • 14 copy number variants (CNVs)
  • 19 fusion variants
  • Across 50 key cancer-related genes

Genes Included in the Oncomine™ Precision Assay

✅ DNA Hotspots

• AKT1
• AKT2
• AKT3
• ALK
• AR
• ARAF
• BRAF
• CDK4
• CDKN2A
• CHEK2
• CTNNB1
• EGFR
• ERBB2
• ERBB3
• ERBB4
• ESR1
• FGFR1
• FGFR2
• FGFR3
• FGFR4
• FLT3
• GNA11
• GNAQ
• GNAS
• HRAS
• IDH1
• IDH2
• KIT
• KRAS
• MAP2K1
• MAP2K2
• MET
• MTOR
• NRAS
• NTRK1
• NTRK2
• NTRK3
• PDGFRA
• PIK3CA
• PTEN
• RAF1
• RET
• ROS1
• SMO
• TP53

✅ CNVs (Copy Number Variants)

• ALK
• AR
• BRAF
• CD274
• EGFR
• ESR1
• FGFR1
• FGFR2
• FGFR3
• KRAS
• MET
• PIK3CA
• PTEN
• TP53

✅ Fusion Variants

• ALK
• AR
• BRAF
• EGFR
• ERBB2
• ESR1
• FGFR1
• FGFR2
• FGFR3
• MET
• NRG1
• NTRK1
• NTRK2
• NTRK3
• NUTM1
• PIK3CA
• RET
• ROS1
• RSPO2
• RSPO3

Test Information

✅ Test Mnemonic:

• OPANGS

For Discovery Health patients only, use the following mnemonics (pre-authorisation code from oncologist required):

• OPALUNG – for lung tumours
• OPACOL – for colorectal tumours

✅ Clinical Indications:

• Oncology patients, particularly those with:
  • Non-small cell lung carcinoma (NSCLC)
  • Colorectal carcinoma (CRC)

✅ Specimen Requirements:

• Formalin-Fixed Paraffin-Embedded (FFPE) Tissue
• Submit:
  • 3–6 normal slides (not charged)
  • Unstained 10-micron thick recuts

✅ Turnaround Time:

• Two weeks (10 working days)

✅ Cost:

• R13,519.00 (2022 cash price)

📧 For more information or to request testing, contact: ngs@ampath.co.za

✅ Let me know when you're ready for the next Lab Update!

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