PATHCHAT Edition
Published: 2024
Please contact your local Ampath pathologist for more information.
What is the Oncomine Comprehensive Assay Plus (OCAPLUS)?
✅ Advanced Solid Tumour Genomic Profiling
- A Next-Generation Sequencing (NGS) test that analyzes over 500 cancer-associated genes.
- Detects:
- Mutations.
- Fusions.
- Copy number variations (CNVs).
✅ Why is OCAPLUS Important?
- Identifies key diagnostic, prognostic, and therapeutic biomarkers.
- Consolidates multiple tests into a single, cost-effective platform.
📌 OCAPLUS enables comprehensive molecular profiling of solid tumours, guiding targeted therapy decisions.
How Does OCAPLUS Differ from the Oncomine Precision Assay (OPANGS)?
✅ Expanded Biomarker Detection
- OPANGS: Covers ~50 tumour-associated genes.
- OCAPLUS: Covers 500+ genes.
✅ Additional Functional Components in OCAPLUS
✔ Component A:
- Detects mutations, fusions, and CNVs for solid tumour diagnosis and treatment.
✔ Component B:
- Predicts response to PARP inhibitors by detecting:
- Homologous recombination deficiency (HRD).
- BRCA1/2 mutations.
- Genomic instability.
✔ Component C:
- Predicts response to immune checkpoint inhibitors by detecting:
- Tumour mutational burden (TMB).
- Microsatellite instability (MSI).
📌 OCAPLUS offers broader tumour profiling, including HRD and immunotherapy response prediction, making it more comprehensive than OPANGS.
How to Request OCAPLUS Testing
✅ Ordering the Test
- Contact the relevant Histopathology laboratory where the case was reported.
- Specify whether you need:
- Full OCAPLUS panel.
- Tumour mutational burden (TMB) only.
✅ Turnaround Time
- Results available within 10–14 working days.
✅ Contact for More Information
- Phone: 012 678 0645
- Email: ngs@ampath.co.za
📌 OCAPLUS testing is a powerful tool for guiding precision oncology treatments.
Key Cancer Types and Relevant Genes in OCAPLUS Panel
✅ Breast, Ovarian, and Endometrial Cancer
- ATM, BARD1, BRCA1, BRCA2, CDK12, CHEK2, ERBB2, FANCD2, PALB2, PPP2RA, TP53, RAD54L, PIK3CA, ESR1.
✅ Colorectal Cancer
- BRAF, ERBB2, KRAS, NRAS, PIK3CA, PTEN.
✅ Liver Cancer
- ARID1A, ARID2, AXIN1, CCND1, CDKN2A, CTNNB1, IDH1, IDH2, JAK1, KEAP, KRAS, MYC, TP53.
✅ Prostate Cancer
- AKT1, APC, AR, BRAF, BRCA2, CDK12, CTNNB1, ERG, FOXA1, HRAS, IDH1, PIK3CA, PTEN, RB1, TP53.
✅ Kidney Cancer
- ATM, BAP1, KDM5C, MET, MTOR, NF2, PBRM1, PTEN, SETD2, TP53, TSC1, TSC2.
✅ Lung Cancer
- AKT1, ALK, BRAF, CTNNB1, DDR2, EGFR, ERBB2, ERBB4, FGFR1, FGFR2, FGFR3, KRAS, PIK3CA, PTEN, TP53.
✅ Melanoma
- AKT3, ARID2, BRAF, CDK4, CDKN2A, CTNNB1, EZH2, GNA11, GNAQ, HRAS, IDH1, KIT, NRAS, RAC1, TP53.
✅ Lymphoma
- ARID1A, ATM, B2M, BCL2, BCL6, BRAF, BTK, CARD11, CD79B, CDKN2A, CREBBP, EZH2, MYD88, TP53.
✅ Central Nervous System (CNS) Tumours
- APC, ATRX, CDKN2A, EGFR, H3F3A, IDH1, IDH2, TP53, TERT.
📌 OCAPLUS provides a broad genetic analysis for multiple solid tumour types, assisting in precise treatment planning.
Conclusion
✅ OCAPLUS is a Next-Generation Sequencing (NGS) test that:
- Detects over 500 genes associated with solid tumours.
- Provides information on actionable mutations, fusion genes, and copy number variations.
- Predicts responses to immunotherapy (TMB, MSI) and PARP inhibitors (HRD, BRCA).
- Enables comprehensive tumour profiling for precision oncology.
📌 For detailed tumour profiling and therapy selection, OCAPLUS offers an all-in-one solution.
Contact Information for Test Requests
✅ For Ordering OCAPLUS Testing
- Ampath NGS Laboratory: 012 678 0645
- Email: ngs@ampath.co.za
📌 OCAPLUS is a cutting-edge NGS test that supports personalized cancer treatment by identifying key genetic mutations and therapy targets.